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birt 377  (MedChemExpress)


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    MedChemExpress birt 377
    Birt 377, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/birt 377/product/MedChemExpress
    Average 93 stars, based on 2 article reviews
    birt 377 - by Bioz Stars, 2026-03
    93/100 stars

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    A: Within one hour after oxygen-glucose-deprivation, <t>BIRT377</t> was applied with consecutive cell death measurement after twenty-four hours via propium iodid incorporation (PI). B: After twenty-four hours, quantification of neuronal death in hippocampal CA1–3 region was determined by PI incorporation after application of 5, 10 or 20 μM BIRT377 after OGD and basal treatment (* p < 0,05, ***p < 0.001 vs OGD).n = 26 Error bars indicate SEM. C: Compared to basal conditions, BIRT377 application dose-dependently increases neuronal cell death with accentuation in CA1-regions (representative PI fluorescent images). Dotted lines and annotations (DG = dentate gyrus, CA = cornu ammonis) illustrate anatomical structure of the hippocampus. CA-region in OGD treated slices is not encircled for increased recognition of the PI positive cells in the CA-1 region. Scale bars: C 1 mm.
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    A: Within one hour after oxygen-glucose-deprivation, <t>BIRT377</t> was applied with consecutive cell death measurement after twenty-four hours via propium iodid incorporation (PI). B: After twenty-four hours, quantification of neuronal death in hippocampal CA1–3 region was determined by PI incorporation after application of 5, 10 or 20 μM BIRT377 after OGD and basal treatment (* p < 0,05, ***p < 0.001 vs OGD).n = 26 Error bars indicate SEM. C: Compared to basal conditions, BIRT377 application dose-dependently increases neuronal cell death with accentuation in CA1-regions (representative PI fluorescent images). Dotted lines and annotations (DG = dentate gyrus, CA = cornu ammonis) illustrate anatomical structure of the hippocampus. CA-region in OGD treated slices is not encircled for increased recognition of the PI positive cells in the CA-1 region. Scale bars: C 1 mm.
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    A: Within one hour after oxygen-glucose-deprivation, BIRT377 was applied with consecutive cell death measurement after twenty-four hours via propium iodid incorporation (PI). B: After twenty-four hours, quantification of neuronal death in hippocampal CA1–3 region was determined by PI incorporation after application of 5, 10 or 20 μM BIRT377 after OGD and basal treatment (* p < 0,05, ***p < 0.001 vs OGD).n = 26 Error bars indicate SEM. C: Compared to basal conditions, BIRT377 application dose-dependently increases neuronal cell death with accentuation in CA1-regions (representative PI fluorescent images). Dotted lines and annotations (DG = dentate gyrus, CA = cornu ammonis) illustrate anatomical structure of the hippocampus. CA-region in OGD treated slices is not encircled for increased recognition of the PI positive cells in the CA-1 region. Scale bars: C 1 mm.

    Journal: PLOS ONE

    Article Title: LFA-1: A potential key player in microglia-mediated neuroprotection against oxygen-glucose deprivation in vitro

    doi: 10.1371/journal.pone.0314020

    Figure Lengend Snippet: A: Within one hour after oxygen-glucose-deprivation, BIRT377 was applied with consecutive cell death measurement after twenty-four hours via propium iodid incorporation (PI). B: After twenty-four hours, quantification of neuronal death in hippocampal CA1–3 region was determined by PI incorporation after application of 5, 10 or 20 μM BIRT377 after OGD and basal treatment (* p < 0,05, ***p < 0.001 vs OGD).n = 26 Error bars indicate SEM. C: Compared to basal conditions, BIRT377 application dose-dependently increases neuronal cell death with accentuation in CA1-regions (representative PI fluorescent images). Dotted lines and annotations (DG = dentate gyrus, CA = cornu ammonis) illustrate anatomical structure of the hippocampus. CA-region in OGD treated slices is not encircled for increased recognition of the PI positive cells in the CA-1 region. Scale bars: C 1 mm.

    Article Snippet: LFA-1 function was inhibited by application of the compound BIRT377 (Tocris), BIRT377 were applied in different concentration to native OHCs and in another set of experiments after addition of exogenous microglia on OHCs at different time points.

    Techniques:

    A. Following the external addition of BV-2 microglia, 20 μM BIRT377 was applied either immediately after or six hours after oxygen-glucose deprivation, with cell death measured by PI after twenty-four hours. B: After twenty-four hours, quantification of neuronal death in hippocampal CA1–3 region was determined by PI incorporation after OGD alone, with externally applied BV-2 microglia and BIRT377 after one and six hours (* p < 0,05, ***p < 0.001 vs OGD)n = 27. Error bars indicate SEM. C: Representative PI fluorescence images of hippocampal slices used in B. Scale bars: C 1 mm.

    Journal: PLOS ONE

    Article Title: LFA-1: A potential key player in microglia-mediated neuroprotection against oxygen-glucose deprivation in vitro

    doi: 10.1371/journal.pone.0314020

    Figure Lengend Snippet: A. Following the external addition of BV-2 microglia, 20 μM BIRT377 was applied either immediately after or six hours after oxygen-glucose deprivation, with cell death measured by PI after twenty-four hours. B: After twenty-four hours, quantification of neuronal death in hippocampal CA1–3 region was determined by PI incorporation after OGD alone, with externally applied BV-2 microglia and BIRT377 after one and six hours (* p < 0,05, ***p < 0.001 vs OGD)n = 27. Error bars indicate SEM. C: Representative PI fluorescence images of hippocampal slices used in B. Scale bars: C 1 mm.

    Article Snippet: LFA-1 function was inhibited by application of the compound BIRT377 (Tocris), BIRT377 were applied in different concentration to native OHCs and in another set of experiments after addition of exogenous microglia on OHCs at different time points.

    Techniques: Fluorescence